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| 23.08.2016 | 20:00 | Quick Boys | - | IJsselmeervogels | 0:2 (0:0) | |
| 24.08.2016 | 18:00 | Amsterdamsche FC | - | Kozakken Boys | 1:5 (1:4) | |
| 18:00 | AFC '34 | - | FC Rijnvogels | 2:6 (2:1) | ||
| 18:00 | VV Dongen | - | OFC Oostzaan | 2:1 (0:1) | ||
| 18:00 | Magreb '90 | - | Harkemase Boys | 3:4 (2:2, 3:3, 5:5) i.E. | ||
| 18:00 | SVV Scheveningen | - | GVVV | 5:2 (0:1) | ||
| 18:00 | Quick'20 | - | JVC Cuijk | 1:3 (0:1) | ||
| 18:00 | VV Sint Bavo | - | USV Hercules | 2:0 (0:0) | ||
| 19:30 | DVS'33 | - | AZSV | 4:2 (1:1) | ||
| 19:30 | VV Schaesberg | - | HSC'21 | 2:3 (1:2) | ||
| 19:30 | VV UNA | - | ODIN '59 | 3:2 (1:0) | ||
| 19:30 | RKSV Halsteren | - | VV Capelle | 0:3 (0:0) | ||
| 19:45 | HBS-Craeyenhout | - | Achilles Veen | 2:3 (1:1) | ||
| 19:45 | Quick Den Haag | - | EVV | 0:1 (0:0, 0:0, 0:1) n.V. | ||
| 20:00 | BVV Barendrecht | - | Juliana '31 | 3:1 (1:0) | ||
| 20:00 | RVVH Ridderkerk | - | ASWH Ido-Ambacht | 0:2 (0:1) | ||
| 20:00 | Jodan Boys | - | SVZW | 3:0 (3:0) | ||
| 20:00 | UDI '19/Beter Bed | - | Rijnsburgse Boys | 0:1 (0:1) | ||
| 20:00 | VV OJC Rosmalen | - | vv Groningen | 5:1 (3:0) | ||
| 20:00 | ONS Sneek | - | ASV De Dijk | 1:2 (0:1) | ||
| 20:00 | sv Huizen | - | RKVV Westlandia | 4:3 (2:1, 3:3, 3:3) i.E. |
