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| 13.09.2023 | 13:00 | Getafe CF | - | Dux Logroño | 1:5 (1:1) | |
| 15:00 | Zaragoza CFF | - | CP Cacereño | 2:3 (0:3) | ||
| 16:30 | Friol | - | Málaga CF | 0:1 (0:0) | ||
| 16:45 | CD Parquesol | - | Córdoba CF | 0:2 (0:1) | ||
| 17:00 | Viajes Interrías FF | - | Fundación Albacete | 2:3 (0:3) | ||
| 17:00 | CDE Racing Féminas | - | Granada CF | 1:4 (1:2) | ||
| 18:00 | Torrelodones CF | - | CD Juan Grande | 0:2 (0:0) | ||
| 18:30 | Sárdoma CF | - | AEM Llleida | 0:2 (0:2) | ||
| 18:30 | CD Pradejón | - | Real Oviedo | 1:3 (0:2) | ||
| 19:00 | SD Huesca | - | Espanyol Barcelona | 0:1 (0:0) | ||
| 19:00 | Bizkerre | - | Rayo Vallecano | 0:2 (0:1) | ||
| 19:00 | CE Europa | - | SD Eibar | 0:2 (0:2) | ||
| 19:30 | Balears FC | - | Deportivo La Coruña | 1:0 (1:0) | ||
| 20:00 | UD Almería | - | CF La Solana | 6:7 (0:0, 0:0) i.E. | ||
| 20:00 | CFF Albacete | - | Real Unión de Tenerife | 0:2 (0:0) | ||
| 21:00 | Fundación Tenerife | - | CA Osasuna | 0:3 (0:2) |
